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complete mouse endothelial cell medium  (Biotrend Chemicals)

 
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    Biotrend Chemicals complete mouse endothelial cell medium
    Complete Mouse Endothelial Cell Medium, supplied by Biotrend Chemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complete+mouse+endothelial+cell+medium/complete+mouse+endothelial+cell+medium/pm37174657-109-6-10
    Average 90 stars, based on 1 article reviews
    complete mouse endothelial cell medium - by Bioz Stars, 2026-10
    90/100 stars

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    Cell Culture:

    Article Title: Deletion of Cd44 Inhibits Metastasis Formation of Liver Cancer in Nf2 -Mutant Mice.
    Article Snippet: C57BL/6 mouse primary lung microvascular endothelial cells were from Biotrend Chemikalien GmbH (Köln, Germany). .. The latter were cultured in complete mouse endothelial cell medium (Biotrend Chemikalien GmbH, Köln, Germany) on plates coated with gelatin (Sigma-Aldrich, Taufkirchen, Germany). ..



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    a Ai9 mice were intravenously injected with 1 ×10 12 gc Cre mRNA containing original VLP (SFV-Env, n = 3) or engineered VLP (Env-P11, n = 4). Scale representing relative levels of fluorescence from high to low (yellow to red). b Liver tdTomato expression was examined by immunofluorescence (tdTomato, green; DAPI, blue; scale bar = 50 μm). c Calculation of the ratio of positive tdTomato cells in different organs. Data represented the mean with standard deviation (SD) of three independent animals and three fields per organ (Brain: PBS vs. SFV-Env, ** P = 0.0068; PBS vs. Env-P11 and SFV-Env vs. Env-P11, **** P < 0.0001. Liver: PBS vs. Env-P11, ** P = 0.0088; PBS vs. SFV-Env and SFV-Env vs. Env-P11, **** P < 0.0001. Spleen: PBS vs. SFV-Env and SFV-Env vs. Env-P11, **** P < 0.0001; two-way ANOVA with Bonferroni’s multiple comparison test). Co-immunofluorescence staining of tdTomato with d neuronal marker NeuN or e glial marker Gfap. (NeuN or Gfap, red; tdTomato, white; DAPI, blue; scale bar = 50 μm). Binding ability of VLP vectors to mouse neural cell lines f N2a (**** P < 0.0001), g bEnd.3 (**** P < 0.0001), and h C8-D1A (**** P < 0.0001), and primary cells i primary pericyte (** P = 0.0046), j primary <t>microvascular</t> <t>endothelial</t> cell (EC) (* P = 0.0429), and k primary astrocyte (*** P = 0.0001), with vector genomic copies per cell of 100,000, normalized using Gapdh . Error bars represented the mean with SD of four independent replicates. Statistical analyses were performed using two-tailed unpaired t test. l Construction of blood-brain barrier spheroids using primary mouse brain microvascular endothelial cells, primary brain microvascular pericytes, and primary astrocytes , . Spheroids were co-incubated with VLPs containing luciferase mRNA at vector genomic copies per cell of m 10,000,000 or n 30,000,000, and luminescence levels of the BBB spheroids were measured. Error bars represented the mean with SD of three independent replicates (* P = 0.0291, two-tailed unpaired t test). Source data are provided as a Source Data file.
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    a Ai9 mice were intravenously injected with 1 ×10 12 gc Cre mRNA containing original VLP (SFV-Env, n = 3) or engineered VLP (Env-P11, n = 4). Scale representing relative levels of fluorescence from high to low (yellow to red). b Liver tdTomato expression was examined by immunofluorescence (tdTomato, green; DAPI, blue; scale bar = 50 μm). c Calculation of the ratio of positive tdTomato cells in different organs. Data represented the mean with standard deviation (SD) of three independent animals and three fields per organ (Brain: PBS vs. SFV-Env, ** P = 0.0068; PBS vs. Env-P11 and SFV-Env vs. Env-P11, **** P < 0.0001. Liver: PBS vs. Env-P11, ** P = 0.0088; PBS vs. SFV-Env and SFV-Env vs. Env-P11, **** P < 0.0001. Spleen: PBS vs. SFV-Env and SFV-Env vs. Env-P11, **** P < 0.0001; two-way ANOVA with Bonferroni’s multiple comparison test). Co-immunofluorescence staining of tdTomato with d neuronal marker NeuN or e glial marker Gfap. (NeuN or Gfap, red; tdTomato, white; DAPI, blue; scale bar = 50 μm). Binding ability of VLP vectors to mouse neural cell lines f N2a (**** P < 0.0001), g bEnd.3 (**** P < 0.0001), and h C8-D1A (**** P < 0.0001), and primary cells i primary pericyte (** P = 0.0046), j primary <t>microvascular</t> <t>endothelial</t> cell (EC) (* P = 0.0429), and k primary astrocyte (*** P = 0.0001), with vector genomic copies per cell of 100,000, normalized using Gapdh . Error bars represented the mean with SD of four independent replicates. Statistical analyses were performed using two-tailed unpaired t test. l Construction of blood-brain barrier spheroids using primary mouse brain microvascular endothelial cells, primary brain microvascular pericytes, and primary astrocytes , . Spheroids were co-incubated with VLPs containing luciferase mRNA at vector genomic copies per cell of m 10,000,000 or n 30,000,000, and luminescence levels of the BBB spheroids were measured. Error bars represented the mean with SD of three independent replicates (* P = 0.0291, two-tailed unpaired t test). Source data are provided as a Source Data file.
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    a Ai9 mice were intravenously injected with 1 ×10 12 gc Cre mRNA containing original VLP (SFV-Env, n = 3) or engineered VLP (Env-P11, n = 4). Scale representing relative levels of fluorescence from high to low (yellow to red). b Liver tdTomato expression was examined by immunofluorescence (tdTomato, green; DAPI, blue; scale bar = 50 μm). c Calculation of the ratio of positive tdTomato cells in different organs. Data represented the mean with standard deviation (SD) of three independent animals and three fields per organ (Brain: PBS vs. SFV-Env, ** P = 0.0068; PBS vs. Env-P11 and SFV-Env vs. Env-P11, **** P < 0.0001. Liver: PBS vs. Env-P11, ** P = 0.0088; PBS vs. SFV-Env and SFV-Env vs. Env-P11, **** P < 0.0001. Spleen: PBS vs. SFV-Env and SFV-Env vs. Env-P11, **** P < 0.0001; two-way ANOVA with Bonferroni’s multiple comparison test). Co-immunofluorescence staining of tdTomato with d neuronal marker NeuN or e glial marker Gfap. (NeuN or Gfap, red; tdTomato, white; DAPI, blue; scale bar = 50 μm). Binding ability of VLP vectors to mouse neural cell lines f N2a (**** P < 0.0001), g bEnd.3 (**** P < 0.0001), and h C8-D1A (**** P < 0.0001), and primary cells i primary pericyte (** P = 0.0046), j primary <t>microvascular</t> <t>endothelial</t> cell (EC) (* P = 0.0429), and k primary astrocyte (*** P = 0.0001), with vector genomic copies per cell of 100,000, normalized using Gapdh . Error bars represented the mean with SD of four independent replicates. Statistical analyses were performed using two-tailed unpaired t test. l Construction of blood-brain barrier spheroids using primary mouse brain microvascular endothelial cells, primary brain microvascular pericytes, and primary astrocytes , . Spheroids were co-incubated with VLPs containing luciferase mRNA at vector genomic copies per cell of m 10,000,000 or n 30,000,000, and luminescence levels of the BBB spheroids were measured. Error bars represented the mean with SD of three independent replicates (* P = 0.0291, two-tailed unpaired t test). Source data are provided as a Source Data file.
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    complete mouse endothelial cell culture medium  (Bioarray Inc)
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    a Ai9 mice were intravenously injected with 1 ×10 12 gc Cre mRNA containing original VLP (SFV-Env, n = 3) or engineered VLP (Env-P11, n = 4). Scale representing relative levels of fluorescence from high to low (yellow to red). b Liver tdTomato expression was examined by immunofluorescence (tdTomato, green; DAPI, blue; scale bar = 50 μm). c Calculation of the ratio of positive tdTomato cells in different organs. Data represented the mean with standard deviation (SD) of three independent animals and three fields per organ (Brain: PBS vs. SFV-Env, ** P = 0.0068; PBS vs. Env-P11 and SFV-Env vs. Env-P11, **** P < 0.0001. Liver: PBS vs. Env-P11, ** P = 0.0088; PBS vs. SFV-Env and SFV-Env vs. Env-P11, **** P < 0.0001. Spleen: PBS vs. SFV-Env and SFV-Env vs. Env-P11, **** P < 0.0001; two-way ANOVA with Bonferroni’s multiple comparison test). Co-immunofluorescence staining of tdTomato with d neuronal marker NeuN or e glial marker Gfap. (NeuN or Gfap, red; tdTomato, white; DAPI, blue; scale bar = 50 μm). Binding ability of VLP vectors to mouse neural cell lines f N2a (**** P < 0.0001), g bEnd.3 (**** P < 0.0001), and h C8-D1A (**** P < 0.0001), and primary cells i primary pericyte (** P = 0.0046), j primary <t>microvascular</t> <t>endothelial</t> cell (EC) (* P = 0.0429), and k primary astrocyte (*** P = 0.0001), with vector genomic copies per cell of 100,000, normalized using Gapdh . Error bars represented the mean with SD of four independent replicates. Statistical analyses were performed using two-tailed unpaired t test. l Construction of blood-brain barrier spheroids using primary mouse brain microvascular endothelial cells, primary brain microvascular pericytes, and primary astrocytes , . Spheroids were co-incubated with VLPs containing luciferase mRNA at vector genomic copies per cell of m 10,000,000 or n 30,000,000, and luminescence levels of the BBB spheroids were measured. Error bars represented the mean with SD of three independent replicates (* P = 0.0291, two-tailed unpaired t test). Source data are provided as a Source Data file.
    Complete Mouse Endothelial Cell Culture Medium, supplied by Bioarray Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complete+mouse+endothelial+cell+medium/human+endothelial+serum+free+medium/pm36302650-156-12-18
    Average 90 stars, based on 1 article reviews
    complete mouse endothelial cell culture medium - by Bioz Stars, 2026-10
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    Image Search Results


    a Ai9 mice were intravenously injected with 1 ×10 12 gc Cre mRNA containing original VLP (SFV-Env, n = 3) or engineered VLP (Env-P11, n = 4). Scale representing relative levels of fluorescence from high to low (yellow to red). b Liver tdTomato expression was examined by immunofluorescence (tdTomato, green; DAPI, blue; scale bar = 50 μm). c Calculation of the ratio of positive tdTomato cells in different organs. Data represented the mean with standard deviation (SD) of three independent animals and three fields per organ (Brain: PBS vs. SFV-Env, ** P = 0.0068; PBS vs. Env-P11 and SFV-Env vs. Env-P11, **** P < 0.0001. Liver: PBS vs. Env-P11, ** P = 0.0088; PBS vs. SFV-Env and SFV-Env vs. Env-P11, **** P < 0.0001. Spleen: PBS vs. SFV-Env and SFV-Env vs. Env-P11, **** P < 0.0001; two-way ANOVA with Bonferroni’s multiple comparison test). Co-immunofluorescence staining of tdTomato with d neuronal marker NeuN or e glial marker Gfap. (NeuN or Gfap, red; tdTomato, white; DAPI, blue; scale bar = 50 μm). Binding ability of VLP vectors to mouse neural cell lines f N2a (**** P < 0.0001), g bEnd.3 (**** P < 0.0001), and h C8-D1A (**** P < 0.0001), and primary cells i primary pericyte (** P = 0.0046), j primary microvascular endothelial cell (EC) (* P = 0.0429), and k primary astrocyte (*** P = 0.0001), with vector genomic copies per cell of 100,000, normalized using Gapdh . Error bars represented the mean with SD of four independent replicates. Statistical analyses were performed using two-tailed unpaired t test. l Construction of blood-brain barrier spheroids using primary mouse brain microvascular endothelial cells, primary brain microvascular pericytes, and primary astrocytes , . Spheroids were co-incubated with VLPs containing luciferase mRNA at vector genomic copies per cell of m 10,000,000 or n 30,000,000, and luminescence levels of the BBB spheroids were measured. Error bars represented the mean with SD of three independent replicates (* P = 0.0291, two-tailed unpaired t test). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Engineering a streamlined virus-like particle for programmable tissue-specific gene delivery

    doi: 10.1038/s41467-025-64181-9

    Figure Lengend Snippet: a Ai9 mice were intravenously injected with 1 ×10 12 gc Cre mRNA containing original VLP (SFV-Env, n = 3) or engineered VLP (Env-P11, n = 4). Scale representing relative levels of fluorescence from high to low (yellow to red). b Liver tdTomato expression was examined by immunofluorescence (tdTomato, green; DAPI, blue; scale bar = 50 μm). c Calculation of the ratio of positive tdTomato cells in different organs. Data represented the mean with standard deviation (SD) of three independent animals and three fields per organ (Brain: PBS vs. SFV-Env, ** P = 0.0068; PBS vs. Env-P11 and SFV-Env vs. Env-P11, **** P < 0.0001. Liver: PBS vs. Env-P11, ** P = 0.0088; PBS vs. SFV-Env and SFV-Env vs. Env-P11, **** P < 0.0001. Spleen: PBS vs. SFV-Env and SFV-Env vs. Env-P11, **** P < 0.0001; two-way ANOVA with Bonferroni’s multiple comparison test). Co-immunofluorescence staining of tdTomato with d neuronal marker NeuN or e glial marker Gfap. (NeuN or Gfap, red; tdTomato, white; DAPI, blue; scale bar = 50 μm). Binding ability of VLP vectors to mouse neural cell lines f N2a (**** P < 0.0001), g bEnd.3 (**** P < 0.0001), and h C8-D1A (**** P < 0.0001), and primary cells i primary pericyte (** P = 0.0046), j primary microvascular endothelial cell (EC) (* P = 0.0429), and k primary astrocyte (*** P = 0.0001), with vector genomic copies per cell of 100,000, normalized using Gapdh . Error bars represented the mean with SD of four independent replicates. Statistical analyses were performed using two-tailed unpaired t test. l Construction of blood-brain barrier spheroids using primary mouse brain microvascular endothelial cells, primary brain microvascular pericytes, and primary astrocytes , . Spheroids were co-incubated with VLPs containing luciferase mRNA at vector genomic copies per cell of m 10,000,000 or n 30,000,000, and luminescence levels of the BBB spheroids were measured. Error bars represented the mean with SD of three independent replicates (* P = 0.0291, two-tailed unpaired t test). Source data are provided as a Source Data file.

    Article Snippet: Primary mouse brain microvascular endothelial cells (Procell, CP-M108) were cultured in Mouse Brain Microvascular Endothelial Cell Complete Medium (Procell, CM-M108).

    Techniques: Injection, Fluorescence, Expressing, Immunofluorescence, Standard Deviation, Comparison, Staining, Marker, Binding Assay, Plasmid Preparation, Two Tailed Test, Incubation, Luciferase